Dawn Chandler S PhD


Dawn Chandler S PhD
Assoc ProfessorCollege of MedicineDawn.Chandler@nationwidechildrens.org
Columbus Children's Research Institute 700 Children's Drive WA5023 Columbus OH 43205
Phone:(614) 722-5302Fax: (614) 722-5895
  • Molecular Biology and Cancer Genetics

General Research Interest

pre-mRNA splicing regulation in cancer and neurodegeneration

Research Description

Our lab is interested in the regulation of pre-mRNA splicing and how disruption of this regulation can lead to pediatric diseases such as cancer.  Current work in our lab elucidates alternative splicing as a novel mechanism by which cellular injury can control the activity of p53 and how changes in the regulation of splicing can lead to tumorigenesis.  The transcription factor p53 is known to induce G1 arrest of the cell cycle and/or apoptosis. MDM2 is one of the most critical regulators of p53.  Using in vitro biochemical assays and genetically engineered mouse models we are currently investigating differential RNA splicing of both the MDM2 and p53 pre-mRNAs and investigating the roles of each in normal cell function as well as disease.

Current Publications

  • Jacob AG, Singh RK, Mohammad F, Bebee TW, Chandler DSThe Splicing Factor FUBP1 Is Required for the Efficient Splicing of Oncogene MDM2 Pre-mRNA.J Biol Chem 289 17350-17364 6/20/2014
  • Chen N, Balasenthil S, Reuther J, Frayna A, Wang Y, Chandler DS, Abruzzo LV, Rashid A, Rodriguez J, Lozano G, Cao Y, Lokken E, Chen J, Frazier ML, Sahin AA, Wistuba II, Sen S, Lott ST, Killary AMDEAR1 is a chromosome 1p35 tumor suppressor and master regulator of TGF-β-driven epithelial-mesenchymal transition.Cancer Discov 3 1172-89 10/1/2013
  • Jacob AG, O'Brien D, Singh RK, Comiskey DF, Littleton RM, Mohammad F, Gladman JT, Widmann MC, Jeyaraj SC, Bolinger C, Anderson JR, Barkauskas DA, Boris-Lawrie K, Chandler DSStress-induced isoforms of MDM2 and MDM4 correlate with high-grade disease and an altered splicing network in pediatric rhabdomyosarcoma.Neoplasia 15 1049-63 9/1/2013
  • Chandler DS, Houghton PJ, London CA, Kraybill W, Perrotti D, Croce CM, Keller C, Guttridge DC, Balkhi MY, Balkhi MY, Iwenofu OH, Bakkar N, Ladner KJmiR-29 acts as a decoy in sarcomas to protect the tumor suppressor A20 mRNA from degradation by HuR.Sci Signal 6 ra63 7/30/2013
  • Bebee TW, Dominguez CE, Samadzadeh-Tarighat S, Akehurst KL, Chandler DSHypoxia is a modifier of SMN2 splicing and disease severity in a severe SMA mouse model.Hum Mol Genet 21 4301-13 10/1/2012
  • Bebee TW, Dominguez CE, Chandler DSMouse models of SMA: tools for disease characterization and therapeutic development.Hum Genet 131 1277-93 8/1/2012

The Ohio State University Comprehensive Cancer Center – Arthur G. James Cancer Hospital and Richard J. Solove Research Institute (OSUCCC – James) 300 W. 10th Ave. Columbus, OH 43210 Phone: 1-800-293-5066 | Email: jamesline@osumc.edu