Tissue Feature Quantification Using
Digital Image Analysis
Tissue features can be specifically stained in mounted tissue
sections, digitized and quantified using tissue morphometric software such as
Definiens Tissue Studio. Quantified results can replace visual imprecise
qualitative assessments. Investigators have used this software based approach
to quantify tissue vascular density, specific cell types in tissue, nuclear
size and a variety of other features.
Figure 1: Quantification and location of malignant
tissue in research tissue samples. Sufficient malignant tissue must be present
in tissue collected for research where a variety of studies may be undertaken.
The required malignant tissue quantity may vary depending on research
objectives. Some studies may require microdissection of the malignant tissue to
enrich the sample. The segmented image can be used to evaluate the expectations
from the procured tissue and to plan for microdissection if required (right). 
Figure 2: Biomarker quantification
and segmentation of cell expression of differentially stained target. Oral
mucosa with dysplasia has sustained overexpression of insulin-like Growth
Factor II MRNA-Binding Protein 3 (IMP3). The degree and extent of dysplasia in
oral mucosal epithelium can be quantified by discriminating the amount and
extent of IMP3/COX2 expression (left). During image analysis, different colors
can be assigned to represent the degree of cell expression (right). 
1. David A.
Kellough, Scott H. Hammond, Randal L. Mandt, Jasmine Ramaradjou, David G. Nohle
and Leona W. Ayers, Tissue Morphometrics Using Definiens Tissue Studio 3.5
Facilitates Tissue Based Data Collection and Management, ISBER (International
Society for Biological and Environmental Repositories) 2014 Annual Meeting,
Walt Disney Dolphin Hotel, 1500 Epcot Resorts Blvd (Dolphin) Lake Buena Vista,
Florida 32830 , May 20-24, 2014.
Published Abstract in: Biorpreservation Biobanking. 2014;10(2):217. QAC 21.
1. Mainville, C.
Allen, LW Ayers, J. Hagen, M. Tong, D. Kellough, S. Mallery. Sustained over
expression of insulin-like Growth Factor II MRNA-Binding Protein 3 (IMP3) in
oral epithelial dysplasia: A potential prognostic biomarker of impending
invasion. American Academy of Oral and Maxillofacial Pathology. 2013 Annual Meeting, Hilton Portland &
Executive Tower, Portland, Oregon, June 14-16, 2013.
2. Akgul M, Nohle DG,
Zhao WJ, Baiocchi RA, Sif S, Kellough DA, Hammond SH, Ayers LW. Mantle cell
lymphoma (MCL) variants can be grouped based on tumor cell nuclear size and
Ki67 proliferation index using digital image analysis. 15th Annual Scientific
Meeting OSUCCC – The James, OHIO UNION, The Ohio State University, Columbus,
OH, April 26, 2013.